Purposetargeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113832||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Gibson Cloning
- 5′ sequencing primer CACACAGGAAACAGCTATGAC
- 3′ sequencing primer CGCAACTGTTGGGAAGGGC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This vector is good for targeting experiment in early embryos and mouse cell lines. Viability problem have been observed when generating knock-in mice.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PBS Actb-2A-TIR1-GFP was a gift from Janet Rossant (Addgene plasmid # 113832 ; http://n2t.net/addgene:113832 ; RRID:Addgene_113832)
For your References section:Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. 10.1038/nbt.4166 PubMed 29889212