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Addgene

PCS2+Cas9-mSA
(Plasmid #103882)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103882 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PCS2+
  • Backbone size w/o insert (bp) 4072
  • Total vector size (bp) 8886
  • Modifications to backbone
    none
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    none
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CAS9-MSA
  • Species
    synthetic
  • Insert Size (bp)
    4818
  • Mutation
    none
  • Promoter SP6
  • Tag / Fusion Protein
    • MSA (monomeric streptavidin (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (not destroyed)
  • 3′ cloning site destroyed (unknown if destroyed)
  • 5′ sequencing primer ATTTAGGTGACACTAT
  • 3′ sequencing primer CCTGTGTGAAATTGTTATCC
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    We cloned this gene ourselves. But we used Cas9 sequence from px330 plasmid from add gene and the MSA sequence from pDisplay-mSA-EGFP-TM (Plasmid #39863)
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

non

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PCS2+Cas9-mSA was a gift from Janet Rossant (Addgene plasmid # 103882 ; http://n2t.net/addgene:103882 ; RRID:Addgene_103882)
  • For your References section:

    Efficient generation of targeted large insertions by microinjection into two-cell-stage mouse embryos. Gu B, Posfai E, Rossant J. Nat Biotechnol. 2018 Jun 11. pii: nbt.4166. doi: 10.1038/nbt.4166. 10.1038/nbt.4166 PubMed 29889212