166,461 results

1. pCMV-MMLVgag-3xNES-Cas9

   Plasmid

   #181752

   Purpose

   MMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLV-Gag-3xNES-Cas9 (Spy)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-MMLVgag-3xNES-ABE8e-NG

   Plasmid

   #181754

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e-NG
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-mBATF

   Plasmid

   #34575

   Depositor

   Peter Murray

   Article

   Smith et al J Biol Chem. 2011 Jul 1;286(26):23582-90

   Insert

   BATF (Batf Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pEvolvR-enCas9-PolI3M-TBD

   Plasmid

   #113077

   Purpose

   Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI3M-TBD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK345 (Hygro-P2A-mAID-mClover)

   Plasmid

   #121179

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mAID-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MGAT-SEP-mRuby3

   Plasmid

   #200937

   Purpose

   cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   MGAT2 (MGAT2 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_AncBE4max

   Plasmid

   #112094

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p11-LacY-wtx1

   Plasmid

   #69056

   Purpose

   The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

   Depositor

   Huimin Zhao

   Article

   Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

   Inserts

   ccdB (ccdB )
   lacY (lacY )
   

   Use

   Tags

   A177C

   Expression

   Mutation

   Promoter

   pBAD and pLac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30a-AlkB

   Plasmid

   #79050

   Purpose

   Express wild type AlkB protein in E. coli BL21(DE3)

   Depositor

   Tao Pan

   Article

   Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

   Insert

   AlkB (alkB E.coli)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   A truncated AlkB with deletion of the amino (N)-t…

   Promoter

   T7

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInducer-myc-ERK2-R67S/S153D

    Plasmid

    #213597

    Purpose

    Doxycycline inducible expression of myc-tagged ERK2-R67S/S153D cDNA in mammilian cells

    Depositor

    Channing Der

    Article

    Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Lentiviral

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    R67S, S153D

    Promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaggs-mGold2t

    Plasmid

    #231764

    Purpose

    Expression of mGold2t in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2t
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2t is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available Since

    April 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

    Plasmid

    #216244

    Purpose

    Express AtAFB2.F74A mutant with mCherry tag for AID2

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    AtAFB2.F74A-mCherry
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    F74A mutation

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-Pol-II WT

    Plasmid

    #35175

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZac2.1-GfaABC1D-lck-jGCaMP8f (AAV1)

    Viral Prep

    #176759-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8f (#176759). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8f plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    GfaABC1D

    Tags

    None

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

15. GFP-APEX2-NIK3x

    Plasmid

    #129274

    Purpose

    Expression of APEX2 in the nucleolus

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    GFP-APEX2-NIK3x
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. GluA2 (px330:2-guideCas9)

    Plasmid

    #169420

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-GAL4

    Plasmid

    #24345

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. AiP12918 - pAAV-AiE0459m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2918)

    Plasmid

    #224016

    Purpose

    AiE0459m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICH41766

    Plasmid

    #48018

    Depositor

    Sylvestre Marillonnet

    Article

    Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. px330_Rosa_sgRNA

    Plasmid

    #97007

    Purpose

    Expresses Cas9 and Rosa26 locus specific sgRNA

    Depositor

    Russell Ray

    Article

    Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

    Insert

    Rosa26 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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