168,493 results

1. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

   Plasmid

   #108912

   Purpose

   Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Has Service

   AAV9

   Insert

   ChroME-ST
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a.Tat(48-57)-EGFP

   Plasmid

   #165040

   Purpose

   Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   EGFP
   

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJAK184

   Plasmid

   #167281

   Purpose

   MazF-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Promoter

   Pxyl

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_C_EPHB2

   Plasmid

   #187797

   Purpose

   MAC-tagged gene expression of human EPHB2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   EPHB2 (EPHB2 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-S5E2-GCaMP6f (AAV9)

   Viral Prep

   #135632-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-FLEX-GFP

   Plasmid

   #28304

   Purpose

   Cre-dependent AAV-mediated expression of GFP under the CAG promoter

   Depositor

   Edward Boyden

   Insert

   GFP
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV Puro

   Plasmid

   #68469

   Purpose

   Retroviral plasmid with multiple cloning site and puromycin resistance

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   Puromycin Resistance
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   pGK

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-HA-USP1

   Plasmid

   #22596

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP1 (USP1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD454-SR mouse Alpha-synuclein

   Plasmid

   #89075

   Purpose

   bacterial expression of mouse Alpha-synuclein

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   Alpha-synuclein (Snca Mouse)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 17, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. SpyCatcher003 S49C

    Plasmid

    #133448

    Purpose

    Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003 S49C
    

    Tags

    GSSGS, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Contains S49C mutation

    Promoter

    T7

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYSD061

    Plasmid

    #212915

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV-U6+27-Tornado-F30-Squash-Broccoli

    Plasmid

    #177918

    Purpose

    Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

    Depositor

    Samie Jaffrey

    Article

    Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

    Insert

    Tornado-F30-Squash-Broccoli
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-dCas9-ZIM3-KRAB-BFP

    Plasmid

    #196712

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-KRAB(ZIM3)-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pG108 - K

    Plasmid

    #178039

    Purpose

    Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Insert

    Kanamycin resistance
    

    Expression

    Bacterial

    Mutation

    ErmAM has been replaced with Kanamycin for select…

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW57.1-DUX4-WT

    Plasmid

    #99282

    Purpose

    All-in-one lentivector with tet-inducible DUX4 (wildetype nucleotide sequence)

    Depositor

    Stephen Tapscott

    Article

    Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Syn61Δ3(ev5)

    Bacterial Strain

    #174514

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pHeACT-mEGFP-NLS

    Plasmid

    #205003

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Hypsibius exemplaris, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the actin gene from Hypsibius exemplaris
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pORANGE Cloning template vector

    Plasmid

    #131471

    Purpose

    Cloning template form ORANGE method based knock-in constructs

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJet1.2 ermB cassette

    Plasmid

    #117121

    Purpose

    Erythromycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Erythromycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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