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182,244 results

Showing: 6181 - 6200 of 182244 results

  1. mito NAD+ biosensor

    Plasmid
    #186791
    Purpose
    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria
    Depositor
    Michael Cohen
    Publication
    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d
    Insert
    mito NAD+ biosensor
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    186791-plasmid-map-sequence-id-368060

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  2. GFP-PS9

    Plasmid
    #177944
    Purpose
    Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eGFP (ORF1ab )
    Expression
    Mammalian
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    177944-plasmid-map-sequence-id-348205

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  3. pAAV-CAG-dLight1.3b (AAV9)

    Viral prep
    #125560-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Publication
    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci
    Promoter
    CAG
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral prep
    #137141-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    nEF
    Tags
    EYFP (Flp-dependent)
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid
    #133451
    Purpose
    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection
    Depositor
    Mark Howarth
    Publication
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TfR-sfGFP-myc tag-SpyCatcher003
    Tags
    GSSGS and myc tag
    Expression
    Mammalian
    Mutation
    Contains C20 and A23 mutations that improve plasm…
    Promoter
    CMV
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133451-plasmid-map-sequence-id-267048

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  19. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    107943-plasmid-map-sequence-id-207614

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Showing: 6181 - 6200 of 182244 results