High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns.
Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S
Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. PubMed Article
Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S
Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153210 | pAGM55261 | Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker |
| 153211 | pAGM55273 | Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker |
| 153212 | pAGM55285 | Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker |
| 153213 | pAGM55297 | Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker |
| 153214 | pAGM65879 | Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and Bar selection cassette |
| 153215 | pAGM65881 | Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation |
| 153216 | pAGM65893 | Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette |
| 153217 | pAGM65905 | Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation |
| 153218 | pAGM37443 | Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium. |
| 153219 | pAGM41991 | Level M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium |
| 153220 | pAGM38869 | Level 0 Arabidopsis U6-promoter vector |
| 153221 | pAGM47523 | Level 0 zCas9i vector |
| 153222 | pAGM35171 | Level 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminator |
| 153223 | pICH67131 | Level 1 position 1 containing the Nos promoter, NPTII (Kan resistance), and the Nos terminator |
| 153224 | pAGM51323 | Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator |
| 153225 | pAGM9037 | Template for PCR amplification of the constant part of guide RNAs |
| 153226 | pAGM51559 | Control plasmid for knockout of TRY and CPC genes |
| 153227 | pAGM55361 | Control plasmid for knockout of AP3 gene |