Purpose(Empty Backbone) Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||153217||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerMarillonnet, IPB
- Backbone size (bp) 6674
Vector typePlant Expression, CRISPR
Growth in Bacteria
Growth Strain(s)DH5alpha ; DH10B
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAGM65905 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153217 ; http://n2t.net/addgene:153217 ; RRID:Addgene_153217)
For your References section:High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Grützner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S. Plant Communications, 2020, 100135 10.1016/j.xplc.2020.100135