pAGM55361
(Plasmid
#153227)
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PurposeControl plasmid for knockout of AP3 gene
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Depositing Lab
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Depositing OrganizationLeibniz Institute of Plant Biochemistry (IPB)
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153227 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 153227-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 153227-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepAGM4723
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Backbone manufacturerMarillonnet, IPB
- Backbone size w/o insert (bp) 4824
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCas9, guide RNA for AP3 and FAST marker
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SpeciesSynthetic
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Insert Size (bp)9622
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2020.04.03.023036v1 for bioRxiv preprint.
DNA (Catalog # 153227-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 153227-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAGM55361 was a gift from Sylvestre Marillonnet (Addgene plasmid # 153227 ; http://n2t.net/addgene:153227 ; RRID:Addgene_153227) -
For your References section:
High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Grutzner R, Martin P, Horn C, Mortensen S, Cram EJ, Lee-Parsons CWT, Stuttmann J, Marillonnet S. Plant Commun. 2020 Nov 23;2(2):100135. doi: 10.1016/j.xplc.2020.100135. eCollection 2021 Mar 8. 10.1016/j.xplc.2020.100135 PubMed 33898975
