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pAAV-Ef1a-Con/Fon-GCaMP6F (AAV8)

Viral Prep

#137122-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-GCaMP6F (#137122). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-GCaMP6F plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of GCaMP6f. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Promoter

EF1a

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only
pCuo CA Rac1

Plasmid

#84643

Purpose

constitutively active Rac1 under cumate-inducible promoter in Lentiviral vector. Also expresses RFP and puromycin resistance.

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

Rac1 (RAC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutively Active, Q61L

Promoter

cumate-inducible promoter

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCepsilon.RNAi

Plasmid

#10798

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC epsilon RNAi (PRKCE Human)

Use

RNAi

Expression

Mammalian

Available Since

April 7, 2006

Availability

Academic Institutions and Nonprofits only
pPD005 pcDNA Golden Gate

Plasmid

#138749

Purpose

Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-KRAS4B(G12V)

Plasmid

#35633

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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Halo-mitoTag

Plasmid

#240424

Purpose

Visualization of mitochondria

Depositor

Anna Akhmanova

Article

Pasolli et al J Cell Biol. 2025 Sep 1;224(9):e20250400

Insert

actA

Tags

Halotag

Expression

Mammalian

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pFCIV-CEACAM6

Plasmid

#149303

Purpose

lentiviral packaging vector for CEACAM6 expression.

Depositor

James Fleckenstein

Article

Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

Insert

CEACAM6 (CEACAM6 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

ubiquitin C

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-NbALFA

Plasmid

#159987

Purpose

For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

mCherry-NbALFA

Tags

8histidine-HRV3C protease cleavage site

Expression

Bacterial

Mutation

3GS linker between mCherry and NbALFA

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pS148∙CsR

Plasmid

#197858

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Ap/Cb resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pEG BacMam C term GFP His8

Plasmid

#160681

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

His8 GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pH6-MBP-TEV-BirA

Plasmid

#179694

Purpose

Expresses His6 tagged MBP-BirA fusion protein

Depositor

Mark Arbing

Insert

BirA

Tags

His6-MBP

Expression

Bacterial

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET mCherry LIC cloning vector (u-mCherry)

Plasmid

#29769

Depositor

Scott Gradia

Type

Empty backbone

Tags

mCherry

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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EGFR-FR

Plasmid

#179263

Purpose

Human EGFR C-terminally labeled with FusionRed

Depositor

Jared Toettcher

Article

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

FusionRed

Promoter

SFFV

Available Since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

Plasmid

#216478

Purpose

lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

YAP5SA
tagBFP

Use

Cre/Lox and Lentiviral

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pGG-cTP-TurboID-GFP

Plasmid

#209403

Purpose

Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

Depositor

Rosa Lozano-Durán

Article

Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

Insert

PM-3XHA-TurbOID

Tags

GFP

Expression

Plant

Promoter

Cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Jan. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-Full-Length-MePCE

Plasmid

#113539

Purpose

Produces an N-terminal GST-tagged full length MePCE protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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CMV HaloTag-LgBiT Vector

Plasmid

#236917

Purpose

Mammalian expression vector encoding HaloTag(R)-LgBiT for use in HiBiT cell lines

Depositor

Promega Corporation

Has Service

DNA

Type

Empty backbone

Tags

HaloTag (R)-LgBiT

Expression

Mammalian

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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