Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18841||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerIn vitrogen
- Backbone size w/o insert (bp) 5882
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1209
MutationThe fluorescent protein YPET was added to the amino terminus of amino acids 148-303 from GGA3.
/ Fusion Proteins
- myc (C terminal on backbone)
- His6 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YPET-GGA3 was a gift from Martin Schwartz (Addgene plasmid # 18841 ; http://n2t.net/addgene:18841 ; RRID:Addgene_18841)
For your References section:A fluorescence resonance energy transfer activation sensor for Arf6. Hall B, McLean MA, Davis K, Casanova JE, Sligar SG, Schwartz MA. Anal Biochem. 2008 Mar 15. 374(2):243-9. 10.1016/j.ab.2007.11.032 PubMed 18162163