MLV Gag-RFP
(Plasmid #1814)

• Depositing Lab: Walther Mothes
• Publication: Sherer et al Traffic 2003 Nov;4(11):785-801.

Backbone

• Vector backbone: pmRFP-N1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gag
• Species: MuLV
• Insert Size (bp): 2100
• Entrez Gene: gag (a.k.a. FrMLVgp1)
• Tag / Fusion Protein:
  • RFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Roger Tsien provided mRFP
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry