|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1812||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)2800
Entrez Genegag (a.k.a. FrMLVgp1)
/ Fusion Protein
- CFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site ??? (unknown if destroyed)
- 3′ cloning site ??? (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMDoldGag-Pol provided by Richard Mulligan
Terms and Licenses
This is a CFP-fusion to the amino acids PQ at the C-terminus of the Gag NC domain. This was cloned by replacing Pol with CFP in pMDoldGag-Pol.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MLV Gag-CFP was a gift from Walther Mothes (Addgene plasmid # 1812 ; http://n2t.net/addgene:1812 ; RRID:Addgene_1812)
For your References section:Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W. Traffic 2003 Nov;4(11):785-801. 10.1034/j.1600-0854.2003.00135.x PubMed 14617360