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HIV-1 Gag-mCherry
(Plasmid #85390)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85390 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/Zeo (+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HIV-1 gag
  • Species
    Human immunodeficiency virus 1
  • Insert Size (bp)
    2214
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gag fragment was amplified from p96ZM651gag-opt vector [NIH AIDS Research and Reference Reagent Program] and cloned into pcDNA3.1/Zeo(+) using HindIII and BamHI sites. mCherry fragment was amplified from pRSET-BmCherry (from Dr R. Tsien, University of California, San Diego) and cloned into pcDNA3.1/Zeo(+) using BamHI and XhoI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HIV-1 Gag-mCherry was a gift from Gregory Melikyan (Addgene plasmid # 85390 ; http://n2t.net/addgene:85390 ; RRID:Addgene_85390)
  • For your References section:

    Visualization of retrovirus uptake and delivery into acidic endosomes. Miyauchi K, Marin M, Melikyan GB. Biochem J. 2011 Mar 15;434(3):559-69. doi: 10.1042/BJ20101588. 10.1042/BJ20101588 PubMed 21175427