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(Plasmid #196071)


Item Catalog # Description Quantity Price (USD)
Plasmid 196071 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Hybrigenics Services
  • Backbone size w/o insert (bp) 4595
  • Total vector size (bp) 4967
  • Modifications to backbone
    3xFLAG inserted at C-terminal on backbone, 3xMYC removed from C-terminal on backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Must be transformed into BL21(DE3) bacteria and grown at 28°C for antibody production.
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Promoter T7
  • Tags / Fusion Proteins
    • 6XHis (C terminal on backbone)
    • 3XFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer GAA TTG TGA GCG GAT AAC
  • 3′ sequencing primer CAG CCC TCA TAG TTA GCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Hybrigenics Services

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHEN2-SG4 was a gift from Shankar Balasubramanian (Addgene plasmid # 196071 ; ; RRID:Addgene_196071)
  • For your References section:

    DNA G-Quadruplex Recognition In Vitro and in Live Cells by a Structure-Specific Nanobody. Galli S, Melidis L, Flynn SM, Varshney D, Simeone A, Spiegel J, Madden SK, Tannahill D, Balasubramanian S. J Am Chem Soc. 2022 Dec 9. doi: 10.1021/jacs.2c10656. 10.1021/jacs.2c10656 PubMed 36488193