pSANG10-3F-BG4
(Plasmid #55756)

• Purpose: Expresses BG4 scFv anti-G-quadruplex antibody in bacteria
• Depositing Lab: Shankar Balasubramanian
• Publication: Biffi et al Nat Chem. 2013 Mar;5(3):182-6. doi: 10.1038/nchem.1548. Epub 2013 Jan 20.

Backbone

• Vector backbone: pSANG10-3F
• Backbone manufacturer: John McCafferty
• Backbone size w/o insert (bp): 5438
• Total vector size (bp): 6197
• Modifications to backbone: None
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Must be transformed into BL21(DE3) bacteria and grown at 30 degrees for antibody production.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: BG4
• Alt name: BG4 anti-G-quadruplex scFv antibody
• Species: human phage display antibody from Sanger library
• Insert Size (bp): 759
• Promoter: T7
• Tags / Fusion Proteins:
  • 6xHis (C terminal on backbone)
  • tri-FLAG (C terminal on backbone)
  • pelB leader (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Ncol (not destroyed)
• 3′ cloning site: Notl (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminal primer

Resource Information

• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry