Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity.
Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR
Nat Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 10.1038/s41587-022-01533-6. PubMed Article
Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR
Nat Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 10.1038/s41587-022-01533-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193782 | SpCas9 TadCBEa | Express TadCBEa (with SpCas9) in mammalian cells |
| 193832 | SpCas9 TadCBEb | Express TadCBEb (with SpCas9) in mammalian cells |
| 193834 | SpCas9 TadCBEc | Express TadCBEc (with SpCas9) in mammalian cells |
| 193835 | SpCas9 TadCBEd | Express TadCBEd (with SpCas9) in mammalian cells |
| 193836 | SpCas9 TadCBEe | Express TadCBEe (with SpCas9) in mammalian cells |
| 193837 | SpCas9 TadDE | Express TadDE (with SpCas9) in mammalian cells |
| 193838 | SpCas9 TadCBEa-V106W | Express TadCBEa-V106W (with SpCas9) in mammalian cells |
| 193839 | SpCas9 TadCBEd-V106W | Express TadCBEd-V106W (with SpCas9) in mammalian cells |
| 193840 | eNme2-C TadCBEa | Express TadCBEa (with eNme2-C variant) in mammalian cells |
| 193841 | eNme2-C TadCBEd | Express TadCBEd (with eNme2-C variant) in mammalian cells |
| 193842 | eNme2-C TadDE | Express TadDE (with eNme2-C variant) in mammalian cells |
| 193843 | SpCas9-TadCBEd-ivt-template | Template for in vitro transcription of TadCBEd (with SpCas9) |
| 193844 | SaCas9 TadCBEa | Express TadCBEa (with SaCas9) in mammalian cells |
| 193845 | SaCas9 TadCBEd | Express TadCBEd (with SaCas9) in mammalian cells |
| 193846 | SaCas9 TadDE | Express TadDE (with SaCas9) in mammalian cells |
| 193847 | p2T-cmv-SpCas9-TadCBEa-BlastR | Express TadCBEa in mammalian cells with BlastR selection, Tol2 integration |
| 193848 | p2T-cmv-SpCas9-TadCBEd-BlastR | Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration |
| 193849 | p2T-cmv-SpCas9-TadCBEd-V106W-BlastR | Express TadCBEd-V106W in mammalian cells with BlastR selection, Tol2 integration |
| 193850 | p2T-cmv-SpCas9-TadDE-BlastR | Express TadDE in mammalian cells with BlastR selection, Tol2 integration |