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PurposeExpress TadCBEd (with SpCas9) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193835 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV with a BR322 origin
- Total vector size (bp) 8780
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTadA-CDd-SpCas9 D10A-2xUGI
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Insert Size (bp)5380
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SpCas9 TadCBEd was a gift from David Liu (Addgene plasmid # 193835 ; http://n2t.net/addgene:193835 ; RRID:Addgene_193835) -
For your References section:
Evolution of an adenine base editor into a small, efficient cytosine base editor with low off-target activity. Neugebauer ME, Hsu A, Arbab M, Krasnow NA, McElroy AN, Pandey S, Doman JL, Huang TP, Raguram A, Banskota S, Newby GA, Tolar J, Osborn MJ, Liu DR. Nat Biotechnol. 2022 Nov 10. pii: 10.1038/s41587-022-01533-6. doi: 10.1038/s41587-022-01533-6. 10.1038/s41587-022-01533-6 PubMed 36357719
