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Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST.
Tong Y, Whitford CM, Robertsen HL, Blin K, Jorgensen TS, Klitgaard AK, Gren T, Jiang X, Weber T, Lee SY
Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
125689pCRISPR-cBESTC to T base editor for actinomycetes
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131464pCRISPR-aBESTExpression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes
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