Highly efficient DSB-free base editing for streptomycetes with CRISPR-BEST.
Tong Y, Whitford CM, Robertsen HL, Blin K, Jorgensen TS, Klitgaard AK, Gren T, Jiang X, Weber T, Lee SY
Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23. PubMed Article
Tong Y, Whitford CM, Robertsen HL, Blin K, Jorgensen TS, Klitgaard AK, Gren T, Jiang X, Weber T, Lee SY
Proc Natl Acad Sci U S A. 2019 Oct 8;116(41):20366-20375. doi: 10.1073/pnas.1913493116. Epub 2019 Sep 23. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125689 | pCRISPR-cBEST | C to T base editor for actinomycetes |
| 131464 | pCRISPR-aBEST | Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes |