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pDEST-FRT/T0-GFP-BRCA1
(Plasmid #71116)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71116 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Promoter CMV/TO
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

siRNA resistant sequence: CAGCTACCCTagtATaATc
The BRCA1 insert contains the following mutations (residue numbering relative to the plasmid's open reading frame): P1565L, E1732G, A1836T, K1877R, and S2307G

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-FRT/T0-GFP-BRCA1 was a gift from Daniel Durocher (Addgene plasmid # 71116 ; http://n2t.net/addgene:71116 ; RRID:Addgene_71116)
  • For your References section:

    A mechanism for the suppression of homologous recombination in G1 cells. Orthwein A, Noordermeer SM, Wilson MD, Landry S, Enchev RI, Sherker A, Munro M, Pinder J, Salsman J, Dellaire G, Xia B, Peter M, Durocher D. Nature. 2015 Dec 9. doi: 10.1038/nature16142. 10.1038/nature16142 PubMed 26649820