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SARS-CoV2 Nucleocapsid
(Plasmid #153201)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 153201 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone manufacturer
    Turner and Rupp
  • Backbone size w/o insert (bp) 4352
  • Total vector size (bp) 5565
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nucleocapsid from SARS-CoV-2
  • Alt name
    N (Severe acute respiratory syndrome coronavirus 2)
  • Species
    severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • Insert Size (bp)
    1260
  • GenBank ID
    MT385497.1
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc tag (5X) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construct made by Xiaolei Liu/Klein Lab

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV2 Nucleocapsid was a gift from Peter Klein (Addgene plasmid # 153201 ; http://n2t.net/addgene:153201 ; RRID:Addgene_153201)
  • For your References section:

    Targeting the coronavirus nucleocapsid protein through GSK-3 inhibition. Liu X, Verma A, Garcia G Jr, Ramage H, Lucas A, Myers RL, Michaelson JJ, Coryell W, Kumar A, Charney AW, Kazanietz MG, Rader DJ, Ritchie MD, Berrettini WH, Schultz DC, Cherry S, Damoiseaux R, Arumugaswami V, Klein PS. Proc Natl Acad Sci U S A. 2021 Oct 19;118(42). pii: 2113401118. doi: 10.1073/pnas.2113401118. Epub 2021 Sep 30. 10.1073/pnas.2113401118 PubMed 34593624