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SARS-CoV2 Nucleocapsid
(Plasmid #153201)

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Item Catalog # Description Quantity Price (USD)
Plasmid 153201 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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DNA 153201-D50 50 μg of DNA in Tris buffer $495 *
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DNA 153201-D100 100 μg of DNA in Tris buffer $585 *
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone manufacturer
    Turner and Rupp
  • Backbone size w/o insert (bp) 4352
  • Total vector size (bp) 5565
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nucleocapsid from SARS-CoV-2
  • Alt name
    N (Severe acute respiratory syndrome coronavirus 2)
  • Species
    severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
  • Insert Size (bp)
    1260
  • GenBank ID
    MT385497.1
  • Entrez Gene
    N (a.k.a. GU280_gp10)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc tag (5X) (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construct made by Xiaolei Liu/Klein Lab

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SARS-CoV2 Nucleocapsid was a gift from Peter Klein (Addgene plasmid # 153201 ; http://n2t.net/addgene:153201 ; RRID:Addgene_153201)

  • For your References section:

    Targeting the coronavirus nucleocapsid protein through GSK-3 inhibition. Liu X, Verma A, Garcia G Jr, Ramage H, Lucas A, Myers RL, Michaelson JJ, Coryell W, Kumar A, Charney AW, Kazanietz MG, Rader DJ, Ritchie MD, Berrettini WH, Schultz DC, Cherry S, Damoiseaux R, Arumugaswami V, Klein PS. Proc Natl Acad Sci U S A. 2021 Oct 19;118(42). pii: 2113401118. doi: 10.1073/pnas.2113401118. Epub 2021 Sep 30. 10.1073/pnas.2113401118 PubMed 34593624
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