Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice.
Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H
Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107307CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyAEncoding an SPH activation system
Loading...
107310dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4Encoding dCas9-10xGCN4 driven by dSV40 promoter
Loading...
107311EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyAEncoding scFv-p65-HSF1 driven by EF1α promoter
Loading...
107312AAV-hSyn-mCherry-P2A-Cre-WPREEncoding hSyn-mCherry-Cre
Loading...
107313AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyAEncoding aCamkII-mCherry-Cre
Loading...