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In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice.
Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H
Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107307CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyAEncoding an SPH activation system
107310dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4Encoding dCas9-10xGCN4 driven by dSV40 promoter
107311EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyAEncoding scFv-p65-HSF1 driven by EF1α promoter
107312AAV-hSyn-mCherry-P2A-Cre-WPREEncoding hSyn-mCherry-Cre
107313AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyAEncoding aCamkII-mCherry-Cre

Recombinant Antibodies from Article