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In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice.
Zhou H, Liu J, Zhou C, Gao N, Rao Z, Li H, Hu X, Li C, Yao X, Shen X, Sun Y, Wei Y, Liu F, Ying W, Zhang J, Tang C, Zhang X, Xu H, Shi L, Cheng L, Huang P, Yang H
Nat Neurosci. 2018 Jan 15. pii: 10.1038/s41593-017-0060-6. doi: 10.1038/s41593-017-0060-6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107307CAG-LoxpStopLoxp-NLS-dCas9-HA-NLS-NLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyAEncoding an SPH activation system
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107310dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4Encoding dCas9-10xGCN4 driven by dSV40 promoter
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107311EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyAEncoding scFv-p65-HSF1 driven by EF1α promoter
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107312AAV-hSyn-mCherry-P2A-Cre-WPREEncoding hSyn-mCherry-Cre
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107313AAV-aCamkII-mCherry-P2A-Cre-WPRE-BGH-polyAEncoding aCamkII-mCherry-Cre
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