Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells.
Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D
Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. Epub 2017 Dec 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101920pINDUCER dCas9-TET1CDMutThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
Loading...
101921pINDUCER dCas9-TET1CDThe dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).
Loading...
101923WPXL SOX10 gRNASOX10 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.
Loading...
101924WPXL LEFTY2 gRNALEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.
Loading...