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pINDUCER dCas9-TET1CDMut
(Plasmid #101920)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101920 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pINDUCER21 (ORF-EG)
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 10591
  • Total vector size (bp) 16882
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-TET1CD Mut
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6391
  • Mutation
    Mutated TET1 catalytic domain

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGTAGGCGTGTACGGTGGGAG
  • 3′ sequencing primer TATCAACCACTTTGTACAAGA
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINDUCER dCas9-TET1CDMut was a gift from Danwei Huangfu (Addgene plasmid # 101920 ; http://n2t.net/addgene:101920 ; RRID:Addgene_101920)
  • For your References section:

    TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells. Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D. Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. Epub 2017 Dec 4. 10.1038/s41588-017-0002-y PubMed 29203910