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David Tirrell Lab Plasmids


The David Tirrell Lab has deposited plasmids at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title Authors Publication
Identification of secreted bacterial proteins by noncanonical amino acid tagging. Mahdavi A, Szychowski J, Ngo JT, Sweredoski MJ, Graham RL, Hess S, Schneewind O, Mazmanian SK, Tirrell DA Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 10.1073/pnas.1301740111. Epub 2013 Dec 17.
Cell-specific proteomic analysis in Caenorhabditis elegans. Yuet KP, Doma MK, Ngo JT, Sweredoski MJ, Graham RL, Moradian A, Hess S, Schuman EM, Sternberg PW, Tirrell DA Proc Natl Acad Sci U S A. 2015 Feb 17. pii: 201421567.
Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. Mahdavi A, Hamblin GD, Jindal GA, Bagert JD, Dong C, Sweredoski MJ, Hess S, Schuman EM, Tirrell DA J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 10.1021/jacs.5b08980. Epub 2016 Mar 25.
David Tirrell's Plasmids (unpublished)
ID Plasmid Gene/Insert Vector Type Publication Hidden Extra Search Info  
51401pAM1MetRS (Other)Identification of secreted bacterial proteins by noncanonical amino acid tagging. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 10.1073/pnas.1301740111. Epub 2013 Dec 17. Expresses truncated version of NLL-MetRS from endogenous MetG promoter pQE80 kan metG b2114, ECK2107, JW2101 Add to Cart
62598pKPY514Thr251Gly-EcPheRS (Other)Bacterial ExpressionCell-specific proteomic analysis in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2015 Feb 17. pii: 201421567. Encodes Thr251Gly-EcPheRS Under IPTG-Inducible (PT5) Control pQE-80L-Kan Add to Cart
62599pKPY197Thr412Gly-CePheRS Alpha Subunit (Caenorhabditis elegans)Worm ExpressionCell-specific proteomic analysis in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2015 Feb 17. pii: 201421567. Encodes Thr412Gly-CePheRS Alpha Subunit pPD95.75 Add to Cart
63177pMarsL274GMarsL274G (Mus musculus)Mammalian ExpressionEngineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 10.1021/jacs.5b08980. Epub 2016 Mar 25. This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter. pcDNA3.1+ Mars Metrs, Mtrns Add to Cart
72669pKPY115Thr251Gly-EcPheRS (Other)Bacterial Expression David Tirrell's Plasmids (unpublished) Encodes Thr251Gly-EcPheRS Under Arabinose-Inducible (PBAD) Control pBAD33 Add to Cart
72673pKPY737Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221 (Caenorhabditis elegans)Gateway Entry Clone David Tirrell's Plasmids (unpublished) Encodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone) pDONR221 Add to Cart
72674pKPY738let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3 (Caenorhabditis elegans)Gateway Entry Clone David Tirrell's Plasmids (unpublished) C. elegans let-858 3' UTR (Gateway Entry Clone) pDONR P2R-P3 Add to Cart
89189pMaRSCFlag-L274G-T2A-mCherry (Mus musculus)Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 10.1021/jacs.5b08980. Epub 2016 Mar 25. The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS. pcDNA 3.1 Mars Metrs, Mtrns Add to Cart