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Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes.
Fine EJ, Appleton CM, White DE, Brown MT, Deshmukh H, Kemp ML, Bao G
Sci Rep. 2015 Jul 1;5:10777. doi: 10.1038/srep10777.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58693N-Terminal Split Cas9 with GyrA inteinExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
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58694C-Terminal Split Cas9 with GyrA inteinExpresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
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58695N-Terminal Split Cas9 D10A Nickase with GyrA inteinExpresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
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