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Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection.
Penewit K, Holmes EA, McLean K, Ren M, Waalkes A, Salipante SJ
MBio. 2018 Feb 20;9(1). pii: mBio.00067-18. doi: 10.1128/mBio.00067-18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107191pCN-EF2132tetExpresses EF2132 recombinase for performing recombineering in S. aureus
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107192pCAS9counterExpresses CAS9 and sgRNA for targeted counterselection in S. aureus
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