Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection.
Penewit K, Holmes EA, McLean K, Ren M, Waalkes A, Salipante SJ
MBio. 2018 Feb 20;9(1). pii: mBio.00067-18. doi: 10.1128/mBio.00067-18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
107191pCN-EF2132tetExpresses EF2132 recombinase for performing recombineering in S. aureus
107192pCAS9counterExpresses CAS9 and sgRNA for targeted counterselection in S. aureus