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pCAS9counter
(Plasmid #107192)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107192 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCN-50
  • Backbone size w/o insert (bp) 5750
  • Total vector size (bp) 7788
  • Modifications to backbone
    Addition of EF2132 and tetR. Replacement of cat194 gene with ermC gene from pCN33. Replacement of pT181cop-634ts with E194ts replicon.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Erythromycin selection in S. aureus, Ampicillin selection in E. coli. Temperature sensitive replicon must be maintained at 32 degrees in S. aureus. Stable at 37 degrees in E. coli.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas9
  • Insert Size (bp)
    4297
  • Promoter prab17

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sgRNA
  • Insert Size (bp)
    324
  • Promoter prab17

Cloning Information for Gene/Insert 2

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAS9counter was a gift from Steve Salipante (Addgene plasmid # 107192 ; http://n2t.net/addgene:107192 ; RRID:Addgene_107192)
  • For your References section:

    Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection. Penewit K, Holmes EA, McLean K, Ren M, Waalkes A, Salipante SJ. MBio. 2018 Feb 20;9(1). pii: mBio.00067-18. doi: 10.1128/mBio.00067-18. 10.1128/mBio.00067-18 PubMed 29463653