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Purpose(Empty Backbone) CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 98211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneUnknown
- Backbone size (bp) 10240
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Vector typeBacterial Expression, CRISPR, Synthetic Biology
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Selectable markersChloramphenicol (in S. aureus)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe plasmid causes the E. coli to grow very slowly at 30C and plates may need to be incubated for 24-36 hours in order to see colonies
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Copy numberHigh Copy
Cloning Information
- Cloning method Unknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCasSA was a gift from Quanjiang Ji (Addgene plasmid # 98211 ; http://n2t.net/addgene:98211 ; RRID:Addgene_98211) -
For your References section:
Rapid and Efficient Genome Editing in Staphylococcus aureus by Using an Engineered CRISPR/Cas9 System. Chen W, Zhang Y, Yeo WS, Bae T, Ji Q. J Am Chem Soc. 2017 Mar 15;139(10):3790-3795. doi: 10.1021/jacs.6b13317. Epub 2017 Mar 2. 10.1021/jacs.6b13317 PubMed 28218837
