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pIMAY
(Plasmid #68939)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68939 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

It is prudent to check that the pIMAY is still temperature sensitive in Staph Aureus before starting any cloning. The author found out that it could lose this function while cultured in Ecoli. That's why Ecoli bearing pIMAY MUST be cultured at 37C in Chloramphenicol (10µg/ml).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIMAY was a gift from Tim Foster (Addgene plasmid # 68939 ; http://n2t.net/addgene:68939 ; RRID:Addgene_68939)
  • For your References section:

    Transforming the untransformable: application of direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis. Monk IR, Shah IM, Xu M, Tan MW, Foster TJ. MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. Print 2012. 10.1128/mBio.00277-11 PubMed 22434850