Purpose(Empty Backbone) E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||68939||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5743
Vector typeStaphylococcal allelic exchange vector
Growth in Bacteria
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
It is prudent to check that the pIMAY is still temperature sensitive in Staph Aureus before starting any cloning. The author found out that it could lose this function while cultured in Ecoli. That's why Ecoli bearing pIMAY MUST be cultured at 37C in Chloramphenicol (10µg/ml).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pIMAY was a gift from Tim Foster (Addgene plasmid # 68939 ; http://n2t.net/addgene:68939 ; RRID:Addgene_68939)
For your References section:Transforming the untransformable: application of direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis. Monk IR, Shah IM, Xu M, Tan MW, Foster TJ. MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. Print 2012. 10.1128/mBio.00277-11 PubMed 22434850