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pIMAY*
(Plasmid #121441)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 121441 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIMAY*
  • Backbone size (bp) 5560
  • Vector type
    Escherichia coli/Staphylococcus aureus shuttle vector
  • Selectable markers
    Chloramphenicol (S. aureus)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    This is an Escherichia coli/Staphylococcus aureus shuttle vector. For S. aureus, grow at 28°C with 10 µg/ml Chloramphenicol.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Tim Foster lab, plasmid pIMAY* originated from pIMAY (https://www.addgene.org/68939/ ). Nucleic acid for counter-selector pheS* was synthesized de novo.
  • Terms and Licenses

Depositor Comments

Always check if the pIMAY* plasmid is still temperature sensitive in Staphylococcus aureus before attempting allelic exchange (see accompanying protocol).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIMAY* was a gift from Angelika Grundling (Addgene plasmid # 121441 ; http://n2t.net/addgene:121441 ; RRID:Addgene_121441)
  • For your References section:

    Use of the counter selectable marker PheS* for genome engineering in Staphylococcus aureus. Schuster CF, Howard SA, Grundling A. Microbiology. 2019 Apr 3. doi: 10.1099/mic.0.000791. 10.1099/mic.0.000791 PubMed 30942689