Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #121441)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 121441 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size (bp) 5560
  • Vector type
    Escherichia coli/Staphylococcus aureus shuttle vector
  • Selectable markers
    Chloramphenicol (S. aureus)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    This is an Escherichia coli/Staphylococcus aureus shuttle vector. For S. aureus, grow at 28°C with 10 µg/ml Chloramphenicol.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Tim Foster lab, plasmid pIMAY* originated from pIMAY ( ). Nucleic acid for counter-selector pheS* was synthesized de novo.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Always check if the pIMAY* plasmid is still temperature sensitive in Staphylococcus aureus before attempting allelic exchange (see accompanying protocol).

This plasmid has been found to be somewhat unstable and prone to concatenation. Concatenation often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIMAY* was a gift from Angelika Grundling (Addgene plasmid # 121441 ; ; RRID:Addgene_121441)
  • For your References section:

    Use of the counter selectable marker PheS* for genome engineering in Staphylococcus aureus. Schuster CF, Howard SA, Grundling A. Microbiology. 2019 Apr 3. doi: 10.1099/mic.0.000791. 10.1099/mic.0.000791 PubMed 30942689