Purpose(Empty Backbone) Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113347||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 17653
Vector typeBacterial Expression, CRISPR, Synthetic Biology
Growth in Bacteria
- Cloning method Unknown
Terms and Licenses
- Not Available to Industry
Selectable Marker: Tetracycline; 30 μg/mL in E.coli and 100 μg/mL in Pseudomonas aeruginosa
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCasPA was a gift from Quanjiang Ji (Addgene plasmid # 113347 ; http://n2t.net/addgene:113347 ; RRID:Addgene_113347)
For your References section:CRISPR/Cas9-based Genome Editing in Pseudomonas aeruginosa and Cytidine Deaminase-Mediated Base Editing in Pseudomonas Species. Chen W, Zhang Y, Zhang Y, Pi Y, Gu T, Song L, Wang Y, Ji Q. iScience. 2018 Aug 31;6:222-231. doi: 10.1016/j.isci.2018.07.024. Epub 2018 Aug 1. 10.1016/j.isci.2018.07.024 PubMed 30240613