168,238 results

1. pcDNA3-YFP

   Plasmid

   #13033

   Purpose

   Mammalian expression vector for cloning your gene fused to YFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Yellow fluorescent protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-CMV-NES-jRCaMP1b

   Plasmid

   #63136

   Purpose

   Red fluorescent calcium sensor protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Insert

   NES-jRCaMP1b
   

   Tags

   nuclear export signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-SARS2-Spike

   Plasmid

   #145032

   Purpose

   Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

   Depositor

   Fang Li

   Article

   Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 Spike (S Synthetic)
   

   Tags

   C9

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGL4.10/RSV_SF3B3-Xon_Luciferase

   Plasmid

   #174659

   Purpose

   Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   Xon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Plasmid

   #179115

   Purpose

   Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site
   

   Use

   CRISPR

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV9)

   Viral Prep

   #104491-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   Syn

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1-mGreenLantern

   Plasmid

   #161912

   Purpose

   mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-VAPB

   Plasmid

   #108126

   Purpose

   ER marker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:VAPB (VAPB Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 HA Arf1 ActQ71L

    Plasmid

    #10832

    Depositor

    Thomas Roberts

    Article

    Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

    Insert

    Arf1 Q71L (ARF1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Q71L. Defective in GTP hydrolysis. Constitutive…

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBPGUw

    Plasmid

    #17575

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Tools for neuroanatomy and neurogenetics

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    March 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti PGK Neo DEST (w531-1)

    Plasmid

    #19067

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. SF-SON IRES Tomato

    Plasmid

    #214403

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-EEA1 wt

    Plasmid

    #42307

    Depositor

    Silvia Corvera

    Article

    Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

    Insert

    EEA1 (EEA1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV.TBG.PI.Cre.rBG

    Plasmid

    #107787

    Purpose

    AAV vector expressing Cre from TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    Cre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TBG

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcVpx.myc

    Plasmid

    #213388

    Purpose

    Generate lentiviral vector which increases gene delivery into primary human myeloid cells

    Depositor

    Nathaniel Landau

    Article

    Sunseri et al J Virol. 2011 Jul;85(13):6263-74. doi: 1

    Insert

    SIVmac vpx (vpx Simian Immunodeficiency Virus)
    

    Use

    Lentiviral

    Tags

    myc-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-Con/Fon-sRGECO (AAV8)

    Viral Prep

    #137126-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Fon-sRGECO (#137126). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Fon-sRGECO plasmid DNA. EF1a-driven, Cre and Flp-dependent expression of cytosolic sRGECO calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    EF1a

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

18. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid

    #226105

    Purpose

    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    atf4 (ATF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains uORF1-2 of ATF4, whose stability can ser…

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEGFP-8xbiRhoBAST

    Plasmid

    #196351

    Purpose

    Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-8xbiRhoBAST
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C3-hYAP1

    Plasmid

    #17843

    Depositor

    Marius Sudol

    Article

    Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

    Insert

    YAP (YAP1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    May 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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