Stat2 pBluescript SK (-)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8705||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK(-)
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2600
Entrez GeneSTAT2 (a.k.a. IMD44, ISGF-3, P113, STAT113)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Diagnostic Restriction Digest with HindIII (2.1kb and 3.5kb).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Stat2 pBluescript SK (-) was a gift from Jim Darnell (Addgene plasmid # 8705 ; http://n2t.net/addgene:8705 ; RRID:Addgene_8705)
For your References section:Proteins of transcription factor ISGF-3: one gene encodes the 91-and 84-kDa ISGF-3 proteins that are activated by interferon alpha. Schindler C, Fu XY, Improta T, Aebersold R, Darnell JE Jr. Proc Natl Acad Sci U S A 1992 Aug 15;89(16):7836-9. 10.1073/pnas.89.16.7836 PubMed 1502203