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Sense codon reassignment enables viral resistance and encoded polymer synthesis.
Robertson WE, Funke LFH, de la Torre D, Fredens J, Elliott TS, Spinck M, Christova Y, Cervettini D, Boge FL, Liu KC, Buse S, Maslen S, Salmond GPC, Chin JW
Science. 2021 Jun 4;372(6546):1057-1062. doi: 10.1126/science.abg3029.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
174514Syn61Δ3(ev5)Recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU and prfA genes. Full sequence - Genbank: CP071799.1
174515pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)
174516pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
174517pBAD_Ub11TCARecoded ubiquitin reporter with TCA replacing codon 11 of wt ubiquitin
174518pBAD_sfGFP3TCG4TAGRecoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

Recombinant Antibodies from Article