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pTriEx-mCherry-Zdk1-TIAM1 DH/PH

Plasmid

#81129

Purpose

mammalian expression of Zdk1 tagged Tiam1 Dh/PH

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Inserts

Zdk1
TIAM1 DH/PH (TIAM1 Human)

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PDCD1LG2-COMP5AP-AviTag-9xHis

Plasmid

#157176

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PDCD1LG2 (PDCD1LG2 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-PDCD1-COMP5AP-AviTag-9xHis

Plasmid

#157108

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

PDCD1 (PDCD1 Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKDM

Plasmid

#110740

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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Sema6a.a-AP-His

Plasmid

#72037

Purpose

Expresses the extracellular region of the Sema6A, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6a.a (Sema6a Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-GFPplus

Plasmid

#172718

Purpose

Expression of a fast folding GFP variant GFP plus

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

GFP plus

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-LK03

Plasmid

#206512

Purpose

AAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Lisowski et al Nature. 2014 Feb 20;506(7488):382-6. doi

Insert

LK03 capsid

Use

AAV

Tags

Expression

Mutation

Promoter

p5

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.28H.28TM.28.3z

Plasmid

#200678

Purpose

Modular CD28hinge-CD28transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD28HD-CD28TM-CD28-CD3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Mutation

Promoter

EF1a-short

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-S4-UD-mCherry

Plasmid

#140306

Purpose

Expresses HA-tagged human Src-S4-UD-mCherry in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src deletion mutant (SRC Human)

Use

Tags

2X FL

Expression

Mammalian

Mutation

contains only the SH4 and UD regions fused to mCh…

Promoter

CMV

Available since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CRISPR

Plasmid

#160047

Purpose

For cloning sgRNA to knockout a specific gene in piggybac plasmid

Depositor

Xiaojun Lian

Article

Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

Type

Empty backbone

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EFS

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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Incoherent feedforward (IFF) plasmid

Plasmid

#169748

Purpose

Standalone IFF circuit plasmid that has the same implementation of the IFF subcircuit in Equalizer-L.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

miR(FF4) target-eGFP-miR(FF4) target-miR(FF4)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV-tetO2

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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TAPBPL pmRFP-N2 (721)

Plasmid

#62000

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

TAPBPL (TAPBPL Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Sema6b(L)-AP-His

Plasmid

#72039

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6c-AP-His

Plasmid

#72041

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMM585

Plasmid

#68884

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Christopher Voigt, Timothy Lu

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pTK25

Plasmid

#37407

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Plk1 (PLK1 Human)

Use

Tags

Mem-mCherry, S-tag, and TEV

Expression

Mammalian

Mutation

K82R

Promoter

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pENTR-L3-SpCas9-HF1-2A-EGFP-L2

Plasmid

#141048

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

SpCas9-HF1-2A-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.22-PGK1-HRE::dLUC

Plasmid

#128095

Purpose

Hypoxia response element real-time luciferase reporter

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)

Use

Luciferase

Tags

drives expression of destabilized luciferase (2CP…

Expression

Mammalian

Mutation

Promoter

inserted PGK1 hypoxia response elements (HRE) (x3)

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GFP-AURKA_K162M-mCherry

Plasmid

#99881

Purpose

Expression of GFP-AURKA K162M-mCherry for protein purification in bacteria

Depositor

Marc Tramier

Article

Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

Insert

AURKA (AURKA Human)

Use

Tags

GFP and mCherry

Expression

Bacterial

Mutation

K162M (kinase-dead)

Promoter

Available since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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