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A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair.
Britton S, Coates J, Jackson SP
J Cell Biol. 2013 Jul 29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46954pAAV-MCS2Cloning plasmid for the generation of Knock-In in human cells using rAAV.
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46955pAAV-Ku70-TCPlasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.
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46956pEGFP-C1-FLAGPlasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.
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46957pEGFP-C1-FLAG-Ku70Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.
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46958pEGFP-C1-FLAG-Ku80Plasmid for expression of GFP-FLAG tagged human Ku80 (N-terminal tag). Confers resistance to G418.
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46959pEGFP-C1-FLAG-XRCC4Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.
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46960pICEPlasmid for cloning and constitutive or doxycycline-inducible expression of cDNAs. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
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46961pICE-EGFP-FLAG-Ku70siR-WTPlasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
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46962pICE-EGFP-FLAG-Ku70siR-Mut6EFor constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
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46963pICE-HA-NLS-I-PpoIFor constitutive or doxycycline-inducible expression of the site specific homing nuclease I-PpoI (human codon optimized). Confers resistance to puro. Use T-REx cells for doxy-inducible expression.
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Antibodies from Article