|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46954||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAgilent Technologies
- Backbone size (bp) 4650
Modifications to backboneA 1,345-bp fragment of Ku80 cDNA was digested with NotI–Bsp12OIand cloned as a linker flanked by restrictions sites into NotI-digested pAAV-MCS.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that the Addgene verified sequence found an 11bp deletion in one ITR and D158G in the Ku80 sequence. The depositor confirmed that this does not impact function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-MCS2 was a gift from Steve Jackson (Addgene plasmid # 46954 ; http://n2t.net/addgene:46954 ; RRID:Addgene_46954)
For your References section:A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. Britton S, Coates J, Jackson SP. J Cell Biol. 2013 Jul 29. 10.1083/jcb.201303073 PubMed 23897892