PurposeAdeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||110660||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 21845
- Total vector size (bp) 21842
Modifications to backboneThe packaging/helper plasmid pDGM6, which includes the serotype 6 capsid reading frame, was generated by amplifying the capsid genefrom a clone of AAV serotype 6 by PCR using the oligonucleotide primers 5′- ATTTAAATCAGGTATGGCTGCCGATGGT-3′ and 5′- ATCGATTGCTATGGTGACCAGATAAGATAA-3′ encoding terminal SwaI and ClaI restriction sites and inserting the resulting DNA fragment into plasmid pDG cut with the same enzymes.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)DH5alpha ; DH10B
Copy numberHigh Copy
Gene/Insert nameAAV6 cap genes, AAV2 rep genes, adenovirus helper genes
Speciesadenovirus, adeno-associated virus
- Cloning method Restriction Enzyme
- 5′ cloning site SwaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDGM6 was a gift from David Russell (Addgene plasmid # 110660 ; http://n2t.net/addgene:110660 ; RRID:Addgene_110660)
For your References section:Systemic delivery of genes to striated muscles using adeno-associated viral vectors. Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller DG, Russell DW, Chamberlain JS. Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 25. 10.1038/nm1085 PubMed 15273747