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(Plasmid #110660)


Item Catalog # Description Quantity Price (USD)
Plasmid 110660 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 21845
  • Total vector size (bp) 21842
  • Modifications to backbone
    The packaging/helper plasmid pDGM6, which includes the serotype 6 capsid reading frame, was generated by amplifying the capsid genefrom a clone of AAV serotype 6 by PCR using the oligonucleotide primers 5′- ATTTAAATCAGGTATGGCTGCCGATGGT-3′ and 5′- ATCGATTGCTATGGTGACCAGATAAGATAA-3′ encoding terminal SwaI and ClaI restriction sites and inserting the resulting DNA fragment into plasmid pDG cut with the same enzymes.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    DH5alpha ; DH10B
  • Copy number
    High Copy


  • Gene/Insert name
    AAV6 cap genes, AAV2 rep genes, adenovirus helper genes
  • Species
    adenovirus, adeno-associated virus

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SwaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDGM6 was a gift from David Russell (Addgene plasmid # 110660 ; ; RRID:Addgene_110660)
  • For your References section:

    Systemic delivery of genes to striated muscles using adeno-associated viral vectors. Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller DG, Russell DW, Chamberlain JS. Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 25. 10.1038/nm1085 PubMed 15273747