|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46958||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4766
- Total vector size (bp) 6912
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)2199
Entrez GeneXRCC5 (a.k.a. KARP-1, KARP1, KU80, KUB2, Ku86, NFIV)
- Promoter CMV
/ Fusion Protein
- GFP-FLAG (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CATGGTCCTGCTGGAGTTCGTG
- 3′ sequencing primer GCAAGTAAAACCTCTACAAATGTGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The depositor notes that the D158G point mutation does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-C1-FLAG-Ku80 was a gift from Steve Jackson (Addgene plasmid # 46958 ; http://n2t.net/addgene:46958 ; RRID:Addgene_46958)
For your References section:A new method for high-resolution imaging of Ku foci to decipher mechanisms of DNA double-strand break repair. Britton S, Coates J, Jackson SP. J Cell Biol. 2013 Jul 29. 10.1083/jcb.201303073 PubMed 23897892