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pLV-ER GFP
(Plasmid #80069)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80069 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV MP2
  • Backbone size w/o insert (bp) 6782
  • Total vector size (bp) 7823
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Use low salt LB, 5 grams of NaCl for both LB and LB agar
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER GFP
  • Alt name
    AcGFP SEC61B
  • Alt name
    AcGFP Sec61 translocon beta subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1041
  • GenBank ID
    NM_006808
  • Entrez Gene
    SEC61B
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP derived from Aequorea coerulescens on the NH2 terminus fused to the full length of SEC61B (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ADDGENE
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-ER GFP was a gift from Pantelis Tsoulfas (Addgene plasmid # 80069 ; http://n2t.net/addgene:80069 ; RRID:Addgene_80069)