pLV-ER GFP
(Plasmid #80069)

• Purpose: To express and target GFP into the endoplasmic reticulum using lentivirus
• Depositing Lab: Pantelis Tsoulfas
• Publication: pLV-ER eGFP (unpublished)

Backbone

• Vector backbone: pLV MP2
• Backbone size w/o insert (bp): 6782
• Total vector size (bp): 7823
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Use low salt LB, 5 grams of NaCl for both LB and LB agar
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ER GFP
• Alt name: AcGFP SEC61B
• Alt name: AcGFP Sec61 translocon beta subunit
• Species: H. sapiens (human)
• Insert Size (bp): 1041
• GenBank ID: NM_006808
• Entrez Gene: SEC61B
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP derived from Aequorea coerulescens on the NH2 terminus fused to the full length of SEC61B (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ADDGENE
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry