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pLV-Golgi eGFP
(Plasmid #79809)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79809 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLV MP2
  • Backbone size w/o insert (bp) 6782
  • Total vector size (bp) 7742
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    LB and LB agar plates in low NaCl 5gm/L
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Golgi eGFP
  • Alt name
    GalT1eGFP
  • Alt name
    UDP-Gal:BetaGlcNAc Beta 1,4- Galactosyltransferase, Polypeptide 1 EGFP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    960
  • GenBank ID
    XM_005251440.4
  • Promoter CMV
  • Tag / Fusion Protein
    • Fusion of the first 62 amino acids of the human betaGalT1 gene to the eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-Golgi eGFP was a gift from Pantelis Tsoulfas (Addgene plasmid # 79809 ; http://n2t.net/addgene:79809 ; RRID:Addgene_79809)