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(Plasmid #54467)


Item Catalog # Description Quantity Price (USD)
Plasmid 54467 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Vector type
    CRISPR ; Toxoplasma expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    SAG1 5' UTR
  • Species
    Toxoplasma gondii

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Modified from pMJ920, which contains the CAS9 human codon optimized gene with a NLS and fused to GFP.
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSAG1::CAS9-U6::sgUPRT was a gift from David Sibley (Addgene plasmid # 54467 ; ; RRID:Addgene_54467)
  • For your References section:

    Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9. Shen B, Brown KM, Lee TD, Sibley LD. MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14. 10.1128/mBio.01114-14 PubMed 24825012