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(Plasmid #58528)


Item Catalog # Description Quantity Price (USD)
Plasmid 58528 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pDEST R4-R3 Vector II
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4555
  • Total vector size (bp) 7635
  • Vector type
    Gateway cloning

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy


  • Gene/Insert name
    DHFR cassette
  • Species
    T. gondii
  • Insert Size (bp)
  • Mutation
    pyrimethamine resistant
  • Promoter DHFR

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pDHFR-TS as a template for DHFR cassette. from David Roos, Donald, R. G., and D. S. Roos. 1993. Stable molecular transformation of Toxoplasma gondii: a selectable dihydrofolate reductase-thymidylate synthase marker based on drug-resistance mutations in malaria. Proc Natl Acad Sci U S A 90:11703-7.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUPRT::DHFR-D was a gift from David Sibley (Addgene plasmid # 58528 ; ; RRID:Addgene_58528)
  • For your References section:

    Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9. Shen B, Brown KM, Lee TD, Sibley LD. MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14. 10.1128/mBio.01114-14 PubMed 24825012