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(Plasmid #87259)


Item Catalog # Description Quantity Price (USD)
Plasmid 87259 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2925
  • Total vector size (bp) 9655
  • Vector type
    Toxoplasma expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    Codon optimized for Toxoplasma gondii expression
  • GenBank ID
  • Promoter TgTUB1 378 bp
  • Tags / Fusion Proteins
    • mAID (C terminal on insert)
    • 3HA (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer M13 Rev caggaaacagctatgac
  • 3′ sequencing primer ggcattatacccgtgtgttacg
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Species
    T. gondii
  • Insert Size (bp)
  • Promoter TgDHFR-TS 463 bp

Cloning Information for Gene/Insert 2

  • Cloning method Unknown
  • 5′ sequencing primer gagacgcgtgtctcgtagaa
  • 3′ sequencing primer aaacgagagacgggcagctt
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT was a gift from David Sibley (Addgene plasmid # 87259 ; ; RRID:Addgene_87259)
  • For your References section:

    Plasma Membrane Association by N-Acylation Governs PKG Function in Toxoplasma gondii. Brown KM, Long S, Sibley LD. MBio. 2017 May 2;8(3). pii: e00375-17. doi: 10.1128/mBio.00375-17. 10.1128/mBio.00375-17 PubMed 28465425