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Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9.
Shen B, Brown KM, Lee TD, Sibley LD
MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
54467pSAG1::CAS9-U6::sgUPRTEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
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58528pUPRT::DHFR-Dreplace TgUPRT with DHFR by homologous recombination
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