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pTet-On-TAP
(Plasmid #59017)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 59017 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTet07Sag1mycGFP
  • Backbone manufacturer
    Dominique Soldati Laboratory
  • Backbone size (bp) 4531
  • Modifications to backbone
    Digested with Kpn1/PacI followed by insertion of a Tet regulatable RPS13 promoter (KpnI/MfeI) and multiple cloning site and TAP tag (MfeI/PacI). Performed a 3 fragment ligation of the pieces into the Tet-On-TAP vector.
  • Vector type
    Toxoplasma Expression
  • Promoter RPS13 with Tet Operators Inserted
  • Selectable markers
    Phleomycin
  • Tag / Fusion Protein
    • Calmodulin Binding Peptide fused with a TEV Cleavage site to Protein A-tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTet-On-TAP was a gift from David Sibley (Addgene plasmid # 59017 ; http://n2t.net/addgene:59017 ; RRID:Addgene_59017)
  • For your References section:

    The Toxoplasma pseudokinase ROP5 forms complexes with ROP18 and ROP17 kinases that synergize to control acute virulence in mice. Etheridge RD, Alaganan A, Tang K, Lou HJ, Turk BE, Sibley LD. Cell Host Microbe. 2014 May 14;15(5):537-50. doi: 10.1016/j.chom.2014.04.002. 10.1016/j.chom.2014.04.002 PubMed 24832449