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pLoxP-DHFR-mCherry
(Plasmid #70147)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70147 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pUC19
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 2100
  • Total vector size (bp) 7317
  • Modifications to backbone
    NO
  • Vector type
    Expression in Toxoplasma gondii
  • Selectable markers
    DHFR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a selectable dihydrofolate reductase-thymidylate synthase marker
  • Alt name
    DHFR-TS-mCherry
  • Species
    Toxoplasma gondii
  • Insert Size (bp)
    4586
  • GenBank ID
    XM_002367211.1 XM_002367211.1
  • Promoter DHFR 5' UTR
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Depositor Comments

Kevin Brown and Shaojun Long jointly built the plasmid in David Sibley lab, for CRISPR genetic operations.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLoxP-DHFR-mCherry was a gift from David Sibley (Addgene plasmid # 70147 ; http://n2t.net/addgene:70147 ; RRID:Addgene_70147)
  • For your References section:

    Analysis of non-canonical calcium dependent protein kinases in Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9. Long S, Wang Q, Sibley LD. Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15 10.1128/IAI.01173-15 PubMed 26755159