Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pSAG1::CAS9-U6::sgUPRT Citations (29)

Plasmid Article: Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9.
Shen B, Brown KM, Lee TD, Sibley LD MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
PubMed Journal

Articles Citing pSAG1::CAS9-U6::sgUPRT

Articles
Genetic mapping reveals that sinefungin resistance in Toxoplasma gondii is controlled by a putative amino acid transporter locus that can be used as a negative selectable marker. Behnke MS, Khan A, Sibley LD. Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14. PubMed

Associated Plasmids

Rhoptry Proteins ROP5 and ROP18 Are Major Murine Virulence Factors in Genetically Divergent South American Strains of Toxoplasma gondii. Behnke MS, Khan A, Lauron EJ, Jimah JR, Wang Q, Tolia NH, Sibley LD. PLoS Genet. 2015 Aug 20;11(8):e1005434. doi: 10.1371/journal.pgen.1005434. eCollection 2015 Aug. PubMed
Analysis of non-canonical calcium dependent protein kinases in Toxoplasma gondii by targeted gene deletion using CRISPR/Cas9. Long S, Wang Q, Sibley LD. Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15 PubMed

Associated Plasmids

An improved method for introducing site-directed point mutation into the Toxoplasma gondii genome using CRISPR/Cas9. Sugi T, Kato K, Weiss LM. Parasitol Int. 2016 May 7. pii: S1383-5769(16)30109-X. doi: 10.1016/j.parint.2016.05.002. PubMed
The Past, Present, and Future of Genetic Manipulation in Toxoplasma gondii. Wang JL, Huang SY, Behnke MS, Chen K, Shen B, Zhu XQ. Trends Parasitol. 2016 Jul;32(7):542-53. doi: 10.1016/j.pt.2016.04.013. Epub 2016 May 12. PubMed
Functional characterization of X-prolyl aminopeptidase from Toxoplasma gondii. Yang M, Zheng J, Jia H, Song M. Parasitology. 2016 Sep;143(11):1443-9. doi: 10.1017/S0031182016000986. Epub 2016 May 25. PubMed
Analysis of the virulence determination mechanisms in a local Toxoplasma strain (T.gHB1) isolated from central China. Zhang W, Li L, Xia N, Zhou Y, Fang R, He L, Hu M, Shen B, Zhao J. Parasitol Res. 2016 May 25. PubMed
Characterization of aspartyl aminopeptidase from Toxoplasma gondii. Zheng J, Cheng Z, Jia H, Zheng Y. Sci Rep. 2016 Sep 28;6:34448. doi: 10.1038/srep34448. PubMed
Functional Characterization of Rhoptry Kinome in the Virulent Toxoplasma gondii RH Strain. Wang JL, Li TT, Elsheikha HM, Chen K, Zhu WN, Yue DM, Zhu XQ, Huang SY. Front Microbiol. 2017 Jan 24;8:84. doi: 10.3389/fmicb.2017.00084. eCollection 2017. PubMed
The moving junction protein RON4, although not critical, facilitates host cell invasion and stabilizes MJ members. Wang M, Cao S, DU N, Fu J, Li Z, Jia H, Song M. Parasitology. 2017 Jun 30:1-8. doi: 10.1017/S0031182017000968. PubMed
Translationally controlled tumor protein is required for the fast growth of Toxoplasma gondii and maintenance of its intracellular development. Zheng J, Chen Y, Li Z, Cao S, Zhang Z, Jia H. FASEB J. 2018 Feb;32(2):906-919. doi: 10.1096/fj.201700994R. Epub 2018 Jan 4. PubMed
Toxoplasma gondii Clp family protein: TgClpB1 plays a crucial role in thermotolerance. Cao S, Du N, Chen H, Pang Y, Zhang Z, Zheng J, Jia H. Oncotarget. 2017 Sep 18;8(49):86117-86129. doi: 10.18632/oncotarget.20989. eCollection 2017 Oct 17. PubMed
Conditional Knockdown of Proteins Using Auxin-inducible Degron (AID) Fusions in Toxoplasma gondii. Brown KM, Long S, Sibley LD. Bio Protoc. 2018 Feb 20;8(4). doi: 10.21769/BioProtoc.2728. PubMed
CRISPR-mediated Tagging with BirA Allows Proximity Labeling in Toxoplasma gondii. Long S, Brown KM, Sibley LD. Bio Protoc. 2018 Mar 20;8(6). doi: 10.21769/BioProtoc.2768. PubMed
Toxoplasma Chinese 1 Strain of WH3Deltarop16I/III /gra15II Genetic Background Contributes to Abnormal Pregnant Outcomes in Murine Model. Wang C, Cheng W, Yu Q, Xing T, Chen S, Liu L, Yu L, Du J, Luo Q, Shen J, Xu Y. Front Immunol. 2018 Jun 1;9:1222. doi: 10.3389/fimmu.2018.01222. eCollection 2018. PubMed
Characterization of Two EF-hand Domain-containing Proteins from Toxoplasma gondii. Chang L, Dykes EJ, Li J, Moreno SNJ, Hortua Triana MA. J Eukaryot Microbiol. 2019 Mar;66(2):343-353. doi: 10.1111/jeu.12675. Epub 2018 Aug 16. PubMed
A Lactate Fermentation Mutant of Toxoplasma Stimulates Protective Immunity Against Acute and Chronic Toxoplasmosis. Xia N, Zhou T, Liang X, Ye S, Zhao P, Yang J, Zhou Y, Zhao J, Shen B. Front Immunol. 2018 Aug 10;9:1814. doi: 10.3389/fimmu.2018.01814. eCollection 2018. PubMed
Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presence of a divergent cytochrome c oxidase. Seidi A, Muellner-Wong LS, Rajendran E, Tjhin ET, Dagley LF, Aw VY, Faou P, Webb AI, Tonkin CJ, van Dooren GG. Elife. 2018 Sep 11;7. pii: 38131. doi: 10.7554/eLife.38131. PubMed
Toxoplasma Effector TgIST Targets Host IDO1 to Antagonize the IFN-gamma-Induced Anti-parasitic Response in Human Cells. Bando H, Sakaguchi N, Lee Y, Pradipta A, Ma JS, Tanaka S, Lai DH, Liu J, Lun ZR, Nishikawa Y, Sasai M, Yamamoto M. Front Immunol. 2018 Sep 19;9:2073. doi: 10.3389/fimmu.2018.02073. eCollection 2018. PubMed
Inducible Nitric Oxide Synthase Is a Key Host Factor for Toxoplasma GRA15-Dependent Disruption of the Gamma Interferon-Induced Antiparasitic Human Response. Bando H, Lee Y, Sakaguchi N, Pradipta A, Ma JS, Tanaka S, Cai Y, Liu J, Shen J, Nishikawa Y, Sasai M, Yamamoto M. MBio. 2018 Oct 9;9(5). pii: mBio.01738-18. doi: 10.1128/mBio.01738-18. PubMed
Characterisation of the Toxoplasma gondii tyrosine transporter and its phosphorylation by the calcium-dependent protein kinase 3. Wallbank BA, Dominicus CS, Broncel M, Legrave N, MacRae JI, Staines HM, Treeck M. Mol Microbiol. 2018 Nov 6. doi: 10.1111/mmi.14156. PubMed
Micronemal protein 13 contributes to the optimal growth of Toxoplasma gondii under stress conditions. Ye S, Xia N, Zhao P, Yang J, Zhou Y, Shen B, Zhao J. Parasitol Res. 2019 Mar;118(3):935-944. doi: 10.1007/s00436-018-06197-3. Epub 2019 Jan 11. PubMed
Characterization of the ATP4 ion pump in Toxoplasma gondii. Lehane AM, Dennis ASM, Bray KO, Li D, Rajendran E, McCoy JM, McArthur HM, Winterberg M, Rahimi F, Tonkin CJ, Kirk K, van Dooren GG. J Biol Chem. 2019 Apr 5;294(14):5720-5734. doi: 10.1074/jbc.RA118.006706. Epub 2019 Feb 5. PubMed
The tyrosine transporter of Toxoplasma gondii is a member of the newly defined apicomplexan amino acid transporter (ApiAT) family. Parker KER, Fairweather SJ, Rajendran E, Blume M, McConville MJ, Broer S, Kirk K, van Dooren GG. PLoS Pathog. 2019 Feb 11;15(2):e1007577. doi: 10.1371/journal.ppat.1007577. eCollection 2019 Feb. PubMed
The Toxoplasma Vacuolar H(+)-ATPase Regulates Intracellular pH and Impacts the Maturation of Essential Secretory Proteins. Stasic AJ, Chasen NM, Dykes EJ, Vella SA, Asady B, Starai VJ, Moreno SNJ. Cell Rep. 2019 May 14;27(7):2132-2146.e7. doi: 10.1016/j.celrep.2019.04.038. PubMed
The Vacuolar Zinc Transporter TgZnT Protects Toxoplasma gondii from Zinc Toxicity. Chasen NM, Stasic AJ, Asady B, Coppens I, Moreno SNJ. mSphere. 2019 May 22;4(3). pii: 4/3/e00086-19. doi: 10.1128/mSphere.00086-19. PubMed
Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii. Hunt A, Russell MRG, Wagener J, Kent R, Carmeille R, Peddie CJ, Collinson L, Heaslip A, Ward GE, Treeck M. Elife. 2019 Oct 2;8. pii: 50598. doi: 10.7554/eLife.50598. PubMed
The Sec1/Munc18-like proteins TgSec1 and TgVps45 play pivotal roles in assembly of the pellicle and sub-pellicle network in Toxoplasma gondii. Cao S, Chen H, Liang X, Fu J, Wang S, Zheng J, Zhang Z, Pang Y, Wang J, Shen B, Jia H. Mol Microbiol. 2020 Jan;113(1):208-221. doi: 10.1111/mmi.14411. Epub 2019 Nov 19. PubMed
Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration. Broncel M, Dominicus C, Vigetti L, Nofal SD, Bartlett EJ, Touquet B, Hunt A, Wallbank BA, Federico S, Matthews S, Young JC, Tate EW, Tardieux I, Treeck M. Elife. 2020 Jul 3;9. pii: 57861. doi: 10.7554/eLife.57861. PubMed

If you have published an article using this plasmid, please email us at [email protected] to have your article added to this page.