Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.
Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W
Genetics. 2015 Apr 8. pii: genetics.115.176917.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64237 | pME-Cas9 | expresses codon optimized Cas9 in zebrafish |
| 64239 | pGGDestTol2LC-1sgRNA | destination vector for one U6x:sgRNA cassette |
| 64240 | pGGDestTol2LC-2sgRNA | destination vector for two U6x:sgRNA cassettes |
| 64241 | pGGDestTol2LC-3sgRNA | destination vector for three U6x:sgRNA cassettes |
| 64242 | pGGDestTol2LC-4sgRNA | destination vector for four U6x:sgRNA cassettes |
| 64243 | pGGDestTol2LC-5sgRNA | destination vector for five U6x:sgRNA cassettes |
| 64245 | pU6a:sgRNA#1 | expresses sgRNA under U6a promoter |
| 64246 | pU6a:sgRNA#2 | expresses sgRNA under U6a promoter |
| 64247 | pU6b:sgRNA#3 | expresses sgRNA under U6b promoter |
| 64248 | pU6c:sgRNA#4 | expresses sgRNA under U6c promoter |
| 64249 | pU6d:sgRNA#5 | expresses sgRNA under U6d promoter |
| 64250 | pU6a:sgRNA(tyr) | expresses sgRNA(tyr) under U6a promoter |