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Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.
Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W
Genetics. 2015 Apr 8. pii: genetics.115.176917.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
64237pME-Cas9expresses codon optimized Cas9 in zebrafish
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64239pGGDestTol2LC-1sgRNAdestination vector for one U6x:sgRNA cassette
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64240pGGDestTol2LC-2sgRNAdestination vector for two U6x:sgRNA cassettes
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64241pGGDestTol2LC-3sgRNAdestination vector for three U6x:sgRNA cassettes
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64242pGGDestTol2LC-4sgRNAdestination vector for four U6x:sgRNA cassettes
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64243pGGDestTol2LC-5sgRNAdestination vector for five U6x:sgRNA cassettes
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64245pU6a:sgRNA#1expresses sgRNA under U6a promoter
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64246pU6a:sgRNA#2expresses sgRNA under U6a promoter
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64247pU6b:sgRNA#3expresses sgRNA under U6b promoter
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64248pU6c:sgRNA#4expresses sgRNA under U6c promoter
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64249pU6d:sgRNA#5expresses sgRNA under U6d promoter
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64250pU6a:sgRNA(tyr)expresses sgRNA(tyr) under U6a promoter
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