Purpose(Empty Backbone) expresses sgRNA under U6a promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64246||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAdam Bogdanove, Daniel Voytas lab
- Backbone size (bp) 2568
Modifications to backbonezebrafish U6 promoter (U6a) directs a sgRNA expression. BsaI sites were added on both ends of U6a:sgRNA cassettes by PCR and PCR products were inserted into the ApaI/XbaI sites of pLR-NN from the Golden Gate TALEN kit.
Growth in Bacteria
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pU6a:sgRNA#2 was a gift from Wenbiao Chen (Addgene plasmid # 64246 ; http://n2t.net/addgene:64246 ; RRID:Addgene_64246)
For your References section:Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs. Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess SM, Patton JG, Chen W. Genetics. 2015 Apr 8. pii: genetics.115.176917. 10.1534/genetics.115.176917 PubMed 25855067